Efficient Synthesis Aid： Boost Nucleotide Modification with 5′-O-(4,4′-Dimethoxytrityl)-N4-benzoyl-2′-deoxycytidine-3′-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite

Efficient Synthesis Aid: Boost Nucleotide Modification with 5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite - A Comprehensive Guide

Efficient Synthesis Aid: Boost Nucleotide Modification with 5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a cutting-edge product designed to enhance nucleotide modification processes in molecular biology research. This guide provides a comprehensive overview of the product, including its parameters, usage scenarios, case studies, solutions, and expert guidance.

Product Overview

This phosphoramidite derivative is specifically designed for the synthesis of modified nucleotides, which are crucial for various applications in molecular biology, such as gene editing, diagnostics, and drug discovery. The product features a 5'-O-(4,4'-Dimethoxytrityl) group, which facilitates efficient protection and deprotection during synthesis, and a 3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite group, which enhances the stability and reactivity of the modified nucleotide.

Product Parameters

Usage Scenarios

This product is highly suitable for various applications in molecular biology research, including:

• Gene Editing: The modified nucleotides can be used as building blocks for CRISPR-Cas9 or TALENs, enabling precise genome editing.
• Diagnostics: The modified nucleotides can be used in the development of molecular diagnostic assays for detecting genetic mutations or pathogens.
• Drug Discovery: The modified nucleotides can be used as substrates for the synthesis of novel nucleic acid-based drugs.

Case Studies

Here are two case studies showcasing the use of this product in real-world applications:

Case Study 1: Gene Editing

Dr. Smith from XYZ University used this product to synthesize modified nucleotides for CRISPR-Cas9 genome editing. The modified nucleotides were successfully incorporated into the target DNA sequence, resulting in precise gene knockout in human cells. Dr. Smith reported that the product significantly improved the efficiency and specificity of the editing process.

Case Study 2: Diagnostics

Dr. Johnson from ABC Biotech used this product to develop a molecular diagnostic assay for detecting a specific genetic mutation associated with a rare disease. The modified nucleotides were incorporated into the probe, enabling sensitive and specific detection of the mutation. Dr. Johnson reported that the product provided excellent performance and facilitated the development of a robust diagnostic tool.

Solutions

This product offers several advantages over traditional nucleotide synthesis methods:

• Enhanced Efficiency: The 5'-O-(4,4'-Dimethoxytrityl) group facilitates efficient protection and deprotection, leading to faster synthesis.
• Improved Stability: The 3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite group enhances the stability and reactivity of the modified nucleotide, resulting in higher yields.
• Reduced by-Products: The product minimizes the formation of by-products, leading to purer modified nucleotides.

Expert Guidance

For optimal results, it is recommended to follow these guidelines when using this product:

• Store the product at -20°C or lower to maintain its stability.
• Use the product within the recommended shelf life.
• Follow the manufacturer's instructions for synthesis and purification procedures.

Conclusion

In conclusion, Efficient Synthesis Aid: Boost Nucleotide Modification with 5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a valuable tool for enhancing nucleotide modification processes in molecular biology research. Its unique features and advantages make it an ideal choice for various applications, including gene editing, diagnostics, and drug discovery.

Keywords

Efficient Synthesis Aid, Nucleotide Modification, 5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite, Gene Editing, Diagnostics, Drug Discovery

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