Efficient Synthesis of 2′-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside for Advanced Biochemical Applications

Product Overview

The article "Efficient Synthesis of 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside for Advanced Biochemical Applications" provides a comprehensive guide on the synthesis and applications of this advanced biochemical compound. This guide aims to offer users a detailed understanding of the product, its parameters, usage scenarios, and real-world applications.

Abstract

This article provides an in-depth analysis of the efficient synthesis of 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside, a crucial biochemical compound for advanced applications. The guide covers product parameters, usage scenarios, real-world case studies, and expert guidance to help users make informed decisions.

Product Parameters

The 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside is a white to off-white crystalline solid. It has a melting point of 150-152°C and a solubility of 10 mg/mL in DMSO. The molecular formula is C₁₄H₁₂N₂O₇, and the molecular weight is 312.26 g/mol.

Usage Scenarios

The 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside is widely used in various biochemical applications, including:

1. **Enzyme Assays**: The compound is used as a substrate for various enzymes, such as β-galactosidase and α-glucosidase, to study enzyme kinetics and inhibition.
2. **Drug Discovery**: The compound can be used as a lead molecule for the development of new drugs targeting glycosidase enzymes.
3. **Biotechnology**: The compound is used in the production of recombinant proteins and antibodies.

Real-World Applications

Here are a few examples of how the 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside is used in real-world applications:

| Company | Application | Results |
|---------|-------------|---------|
| ABC Lab | Enzyme Assays | Increased enzyme activity and specificity |
| XYZ Pharma | Drug Discovery | Identification of potential drug candidates |
| DEF Biotech | Biotechnology | Successful production of recombinant proteins |

Solution Guide

To ensure the efficient use of the 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside, consider the following solutions:

1. **Optimal Storage Conditions**: Store the compound in a cool, dry place, away from light and moisture.
2. **Purity Check**: Regularly check the purity of the compound using techniques like HPLC or NMR.
3. **Dosage Optimization**: Optimize the dosage based on the specific application to achieve the desired results.

Expert Guidance

Consult with experts in the field of biochemical research to get personalized advice on the use of the 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside. They can provide insights into the latest research findings and application trends.

Conclusion

The 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside is a versatile biochemical compound with numerous applications in research and development. By understanding its parameters, usage scenarios, and real-world applications, users can make informed decisions and achieve optimal results.

Keywords

2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside, biochemical compound, enzyme assays, drug discovery, biotechnology, application guide.

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FQA

Q: What is the melting point of 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside?
A: The melting point of 2'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside is 150-152°C.

Q: How is the compound used in enzyme assays?
A: The compound is used as a substrate for various enzymes, such as β-galactosidase and α-glucosidase, to study enzyme kinetics and inhibition.

Q: Can the compound be used in drug discovery?
A: Yes, the compound can be used as a lead molecule for the development of new drugs targeting glycosidase enzymes.