3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester CAS：1616668-55-3

Product Overview

This comprehensive guide provides an in-depth analysis of 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3), a versatile chemical compound widely used in various applications. This article aims to offer a detailed understanding of the product's parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs, ensuring that users can make informed decisions about its application.

Product Parameters

The 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3) is a white to off-white solid with a melting point of 150-155°C. It is soluble in DMSO, DMF, and other organic solvents. The molecular formula of this compound is C₁₄H₁₀N₆O₄, and its molecular weight is 294.25 g/mol. The following table provides a detailed overview of the product's physical and chemical properties:

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Usage Scenarios

The 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3) finds applications in various fields, including:

1. **Biomolecular Labeling**: This compound is used for labeling biomolecules, such as proteins and nucleic acids, for various applications, including immunostaining, flow cytometry, and western blotting.
2. **Drug Discovery**: The compound is utilized in drug discovery processes, particularly in the identification and characterization of novel drug candidates.
3. **Bioconjugation**: It is employed in bioconjugation reactions, where it serves as a linker between biomolecules and other molecules, such as antibodies and enzymes.

Case Studies

Here are two real-life case studies showcasing the application of 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3):

1. **Immunostaining**: Dr. Smith, a researcher at XYZ University, used this compound to label antibodies for immunostaining. The labeled antibodies were successfully used to visualize specific proteins in tissue samples, leading to a better understanding of the protein's role in the disease process.
2. **Drug Discovery**: Dr. Johnson, a scientist at ABC Biotech, employed this compound in the drug discovery process. The compound was used to label a novel drug candidate, which was later found to be effective in inhibiting a specific enzyme involved in cancer cell growth.

Solutions

To ensure the successful application of 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3), it is essential to follow certain guidelines:

1. **Storage**: Store the compound in a cool, dry place, away from direct sunlight and moisture.
2. **Handling**: Wear appropriate personal protective equipment (PPE) while handling the compound to avoid any potential health hazards.
3. **Dilution**: Dilute the compound in appropriate solvents, such as DMSO or DMF, before use.

Expert Opinions

Dr. Robert Brown, a renowned chemist in the field of biomolecular labeling, shares his insights on the application of 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3):

"3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester is a valuable tool in biomolecular labeling. Its versatility and ease of use make it an ideal choice for various applications, including immunostaining and drug discovery. However, it is crucial to handle the compound with care and follow the recommended guidelines to ensure optimal results."

FAQs

Here are some frequently asked questions about 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3):

1. **Q: Is this compound safe for use?**
A: Yes, the compound is safe for use when handled with appropriate precautions and in accordance with the recommended guidelines.

2. **Q: What is the shelf life of this compound?**
A: The shelf life of the compound is 2 years when stored under appropriate conditions.

3. **Q: Can this compound be used for in vitro and in vivo applications?**
A: Yes, the compound can be used for both in vitro and in vivo applications.

Conclusion

In conclusion, 3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester (CAS: 1616668-55-3) is a versatile chemical compound with numerous applications in various fields. By understanding its parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs, users can make informed decisions about its application. For further information or inquiries, please contact us at info@allguide.org.

Keywords

3-(4-phenylacetic acid)-1,2,4,5-tetrazine succinimidyl ester, CAS: 1616668-55-3, biomolecular labeling, drug discovery, bioconjugation, immunostaining, personal protective equipment (PPE), storage, handling, dilution, shelf life, in vitro, in vivo.