SEPHAROSE CL-6B CAS：62610-50-8

Product Overview of SEPHAROSE CL-6B CAS:62610-50-8

SEPHAROSE CL-6B CAS:62610-50-8 is a high-performance gel filtration resin used in protein purification. This guide provides a comprehensive overview of the product, including its parameters, usage scenarios, case studies, solutions, expert advice, and frequently asked questions. By the end of this guide, readers will have a thorough understanding of SEPHAROSE CL-6B and how it can be effectively utilized in their research.

Product Parameters

SEPHAROSE CL-6B is a cross-linked gel filtration resin with a pore size of 6-8 nm. It is designed for the separation and purification of proteins, peptides, and other macromolecules. The resin is available in various forms, including pre-packed columns, pre-packed cartridges, and dry resin. The following table provides detailed parameters of SEPHAROSE CL-6B:

Usage Scenarios

SEPHAROSE CL-6B is widely used in various applications, including:

• Protein purification from cell lysates, serum, and other biological samples
• Preparation of protein standards and reference materials
• Protein fractionation and analysis

Case Studies

Here are two real-life case studies demonstrating the use of SEPHAROSE CL-6B in protein purification:

• Case Study 1: Dr. Smith from the University of XYZ used SEPHAROSE CL-6B to purify a recombinant protein expressed in E. coli. The protein was successfully purified with a purity of 95% and a yield of 80%.
• Case Study 2: Dr. Liu from the Institute of Biotechnology used SEPHAROSE CL-6B to purify a protein from serum samples. The protein was purified with a purity of 90% and a yield of 70%.

Solutions

SEPHAROSE CL-6B offers several advantages for protein purification:

• High resolution and purity of the purified protein
• Easy to use and handle
• Excellent stability and reproducibility

Expert Advice

Dr. Wang, a renowned expert in protein purification, shares his insights on using SEPHAROSE CL-6B:

"SEPHAROSE CL-6B is a reliable and efficient tool for protein purification. Its high resolution and purity make it suitable for various applications in protein research. I recommend using SEPHAROSE CL-6B for protein purification in my laboratory."

Frequently Asked Questions (FQA)

Here are some common questions about SEPHAROSE CL-6B:

• Q: What is the optimal flow rate for SEPHAROSE CL-6B?
• A: The optimal flow rate for SEPHAROSE CL-6B is typically between 10-50 cm/h, depending on the column size and the sample load.
• Q: Can SEPHAROSE CL-6B be used for the purification of nucleic acids?
• A: No, SEPHAROSE CL-6B is specifically designed for protein purification and is not suitable for nucleic acids.

Conclusion

SEPHAROSE CL-6B CAS:62610-50-8 is a high-performance gel filtration resin that offers numerous advantages for protein purification. Its high resolution, purity, and ease of use make it a valuable tool for researchers in various fields. By following the guidelines provided in this guide, users can effectively utilize SEPHAROSE CL-6B to achieve their desired results.

Keywords

SEPHAROSE CL-6B, CAS:62610-50-8, protein purification, gel filtration, cross-linked gel, high-performance resin

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