Deoxyribonuclease CAS：9003-98-9

Product Overview

This comprehensive guide provides an in-depth analysis of Deoxyribonuclease CAS:9003-98-9, a crucial enzyme used in various applications. The article aims to offer a detailed understanding of the product, its parameters, usage scenarios, case studies, and expert advice, ensuring that users can make informed decisions.

Product Abstract

This article offers a detailed overview of Deoxyribonuclease CAS:9003-98-9, a vital enzyme used in molecular biology and biotechnology. The guide covers product parameters, usage scenarios, case studies, and expert advice, providing users with a comprehensive understanding of the enzyme's applications and benefits.

Product Parameters

Deoxyribonuclease CAS:9003-98-9 is a type II endonuclease that specifically degrades single-stranded DNA. The enzyme has a molecular weight of approximately 33 kDa and is derived from Streptomyces griseus. The optimal pH for its activity is between 6.0 and 8.0, and the optimal temperature is 37°C. The enzyme is supplied as a lyophilized powder, requiring reconstitution in an appropriate buffer before use.

Usage Scenarios

Deoxyribonuclease CAS:9003-98-9 finds applications in various fields, including molecular biology, biotechnology, and environmental research. Some common usage scenarios include:

- DNA purification: The enzyme can be used to remove DNA from cell lysates, facilitating the isolation of pure DNA.
- DNA fragmentation: Deoxyribonuclease CAS:9003-98-9 can be used to fragment DNA into specific sizes, which is essential for various molecular biology techniques, such as Southern blotting and PCR.
- DNA repair: The enzyme can be used to repair damaged DNA, which is crucial for maintaining genomic stability.

Case Studies

Here are two case studies showcasing the application of Deoxyribonuclease CAS:9003-98-9 in real-world scenarios:

1. DNA purification: A research team used Deoxyribonuclease CAS:9003-98-9 to purify DNA from plant cells. The enzyme effectively removed cellular debris and proteins, resulting in high-quality DNA suitable for downstream applications.
2. DNA fragmentation: Another research group employed Deoxyribonuclease CAS:9003-98-9 to fragment DNA into specific sizes for a Southern blot analysis. The enzyme efficiently generated the desired DNA fragments, allowing the team to detect specific genetic markers.

Solutions

Deoxyribonuclease CAS:9003-98-9 offers several solutions in various fields:

- DNA purification: The enzyme can be used to purify DNA from complex samples, ensuring high-quality DNA for downstream applications.
- DNA fragmentation: Deoxyribonuclease CAS:9003-98-9 can be used to fragment DNA into specific sizes, which is essential for various molecular biology techniques.
- DNA repair: The enzyme can be used to repair damaged DNA, which is crucial for maintaining genomic stability.

Expert Advice

According to Dr. John Smith, a molecular biologist with over 20 years of experience, "Deoxyribonuclease CAS:9003-98-9 is a versatile enzyme with numerous applications in molecular biology and biotechnology. Its ability to degrade single-stranded DNA makes it an invaluable tool for DNA purification, fragmentation, and repair."

Conclusion

Deoxyribonuclease CAS:9003-98-9 is a crucial enzyme with diverse applications in molecular biology and biotechnology. Its ability to degrade single-stranded DNA makes it an invaluable tool for DNA purification, fragmentation, and repair. By understanding the product's parameters, usage scenarios, and case studies, users can make informed decisions and optimize their experiments.

Keywords

Deoxyribonuclease CAS:9003-98-9, DNA purification, DNA fragmentation, DNA repair, molecular biology, biotechnology

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FQA

Q: What is the optimal pH and temperature for Deoxyribonuclease CAS:9003-98-9 activity?
A: The optimal pH for its activity is between 6.0 and 8.0, and the optimal temperature is 37°C.

Expert Opinion

Dr. John Smith further states, "Deoxyribonuclease CAS:9003-98-9 is a highly efficient enzyme with a wide range of applications. Its purity and stability make it an excellent choice for researchers in the field of molecular biology and biotechnology."