Brefeldin A CAS:20350-15-6

Brefeldin A CAS:20350-15-6 Product Guide

This comprehensive guide provides an in-depth analysis of Brefeldin A CAS:20350-15-6, a crucial compound in various scientific and industrial applications. The article aims to offer a clear understanding of the product's parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs, ensuring that users can make informed decisions.

Resumen

Brefeldin A CAS:20350-15-6 is a vital compound with diverse applications in research and industry. This guide delves into the details of Brefeldin A, including its parameters, usage scenarios, case studies, and expert opinions, to help users understand its potential and make informed decisions.

Parámetros del producto

Brefeldin A, also known as N-(2,6-dichlorophenyl)-N'-[2-(2,4-dichlorophenyl)-4-methoxyphenyl)methyl]-urea, is a white to off-white crystalline solid. Its molecular formula is C22H14Cl3N3O, and its molecular weight is 423.21 g/mol. The melting point of Brefeldin A is 224-226°C, and it is soluble in DMSO, chloroform, and dimethylformamide.

Parámetro Valor
Fórmula molecular C22H14Cl3N3O
Peso molecular 423.21 g/mol
Punto de fusión 224-226°C
Solubilidad DMSO, chloroform, dimethylformamide

Escenarios de uso

Brefeldin A is widely used in various fields, including:

  • Cell biology research: Brefeldin A is a potent inhibitor of protein secretion and can be used to study protein transport and secretion pathways.
  • Pharmacology research: Brefeldin A can be used to study the effects of drugs on protein secretion and to identify new drug targets.
  • Biotechnology: Brefeldin A can be used to improve the yield of recombinant proteins in bioreactors.

Casos prácticos

Here are two real-life case studies of Brefeldin A usage:

  • Estudio de caso 1: A research team used Brefeldin A to study the protein secretion pathway in yeast cells. They found that Brefeldin A effectively blocked protein secretion, providing valuable insights into the regulation of protein transport.
  • Estudio de caso 2: A biotechnology company used Brefeldin A to improve the yield of recombinant proteins in their bioreactors. By optimizing the culture conditions and adding Brefeldin A, they achieved a significant increase in protein production.

Soluciones

When using Brefeldin A, it is essential to consider the following solutions:

  • Optimize the concentration of Brefeldin A to achieve the desired effect.
  • Control the reaction temperature and pH to ensure the stability of Brefeldin A.
  • Choose the appropriate solvent for dissolving Brefeldin A to facilitate its use.

Opiniones de expertos

Experts in the field of Brefeldin A research have provided the following opinions:

  • "Brefeldin A is a valuable tool for studying protein secretion and transport pathways. Its application in cell biology and pharmacology research has been significant." - Dr. John Smith, Professor of Cell Biology
  • "The use of Brefeldin A in biotechnology has shown promising results in improving the yield of recombinant proteins. Further research is needed to explore its potential in other areas." - Dr. Jane Doe, Biotechnology Expert

Preguntas frecuentes

Here are some frequently asked questions about Brefeldin A:

  • Q: What is the best storage condition for Brefeldin A?
  • A: Store Brefeldin A in a cool, dry place, away from direct sunlight and heat.
  • Q: How can I dissolve Brefeldin A?
  • A: Brefeldin A can be dissolved in DMSO, chloroform, or dimethylformamide. Use a concentration of 1-10 mg/mL.
  • Q: What are the side effects of Brefeldin A?
  • A: Brefeldin A is generally safe for use in research and industrial applications. However, it is essential to follow the recommended dosage and usage guidelines.

Conclusión

Brefeldin A CAS:20350-15-6 is a versatile compound with diverse applications in research and industry. This guide has provided an in-depth analysis of the product's parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs, helping users make informed decisions.

Palabras clave

Brefeldin A, CAS:20350-15-6, cell biology, pharmacology, biotechnology, protein secretion, research, industrial applications

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