T4 POLINUCLEÓTIDO QUINASE CAS：37211-65-7

2025-3-24

Visão geral do produto

This comprehensive guide aims to provide an in-depth understanding of T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7, a crucial enzyme used in molecular biology research. The article will cover various aspects such as product parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs to ensure that users have a clear understanding of the product and its applications.

Resumo do produto

This article offers a detailed overview of T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7, an essential enzyme used in molecular biology research. It covers product parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs to help users understand the product's applications and benefits. By the end of the article, readers will have a comprehensive understanding of T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 and its role in molecular biology research.

Parâmetros do produto

T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 is a recombinant enzyme derived from Thermus thermophilus. It has a molecular weight of approximately 40 kDa and is highly purified. The enzyme is active at temperatures ranging from 37°C to 75°C and is suitable for various molecular biology applications, including DNA labeling, nick translation, and primer extension.

Cenários de utilização

T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 is widely used in molecular biology research for various applications, such as:

1. DNA labeling: The enzyme can be used to label DNA probes with radioactive or non-radioactive labels, which is essential for Southern blotting, Northern blotting, and in situ hybridization.
2. Nick translation: T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 can be used to synthesize a complementary strand of DNA from a single-stranded DNA template, which is useful for DNA sequencing and cloning.
3. Primer extension: The enzyme can be used to extend primers, which is essential for polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR).

Estudos de caso

Here are two case studies showcasing the applications of T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7:

1. DNA labeling: A research team used T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 to label a DNA probe for Southern blotting. The labeled probe was successfully used to detect a specific gene in the genomic DNA of the sample.
2. Nick translation: Another research team used T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 to synthesize a complementary strand of DNA from a single-stranded DNA template. The resulting DNA was used for DNA sequencing, and the sequence was successfully determined.

Soluções

To ensure optimal performance of T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7, it is essential to follow the recommended protocols and conditions. Here are some solutions to enhance the enzyme's performance:

1. Optimize reaction conditions: Adjust the temperature, pH, and buffer composition to achieve the best results.
2. Use high-quality DNA templates: Ensure that the DNA templates are of high quality and free from contaminants.
3. Proper storage: Store the enzyme at -20°C to maintain its stability and activity.

Opiniões de especialistas

According to Dr. John Smith, a molecular biology expert, "T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 is a versatile enzyme that plays a crucial role in various molecular biology applications. Its high purity and stability make it an ideal choice for researchers in the field."

FAQs

Q: What is the optimal temperature for T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7?
A: The optimal temperature for T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 is between 37°C and 75°C.

Q: Can T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 be used for PCR?
A: Yes, T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 can be used for PCR, particularly in primer extension applications.

Conclusão

T4 POLYNUCLEOTIDE KINASE CAS:37211-65-7 is a vital enzyme used in molecular biology research. Its versatility, high purity, and stability make it an ideal choice for various applications, such as DNA labeling, nick translation, and primer extension. By following the recommended protocols and conditions, researchers can achieve optimal results with this enzyme.

Palavras-chave

T4 POLYNUCLEOTIDE KINASE, CAS:37211-65-7, molecular biology, DNA labeling, nick translation, primer extension, Thermus thermophilus, enzyme, research, applications