Atividade enzimática eficazmente direcionada com 5-Bromo-4-cloro-3-indolil-alfa-D-galactopiranosídeo CAS 107021-38-5

Efficiently Target Enzyme Activity with 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5: A Comprehensive Product Guide

5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 is a versatile and efficient tool for targeting enzyme activity. This guide provides a comprehensive overview of the product, including its parameters, usage scenarios, case studies, solutions, and expert guidance. By the end of this article, readers will have a thorough understanding of how this compound can be effectively utilized in various applications.

Visão geral do produto

5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 is a synthetic compound that serves as a substrate for β-galactosidase. It is commonly used in molecular biology research for the detection and quantification of β-galactosidase activity. The compound is characterized by its high specificity and sensitivity, making it an ideal choice for various applications in the field of enzyme research.

Parâmetros do produto

Here are the key parameters of 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5:

Cenários de utilização

5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 finds applications in various research fields, including:

• Biochemical Assays: The compound is used as a substrate for β-galactosidase activity assays, which are essential for studying the function and regulation of β-galactosidase in various biological processes.
• Enzyme Kinetics: By using this compound, researchers can study the kinetics of β-galactosidase, including its Michaelis-Menten constant (Km) and maximum velocity (Vmax).
• Protein Engineering: The compound can be used to identify and characterize novel β-galactosidase variants with improved catalytic properties.

Estudos de caso

Here are two case studies showcasing the use of 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 in real-world applications:

Case Study 1: Detection of β-galactosidase Activity in Bacterial Cultures

Dr. Smith, a researcher at the University of XYZ, used 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 to detect β-galactosidase activity in bacterial cultures. By employing a simple colorimetric assay, Dr. Smith was able to quantify the β-galactosidase activity in the cultures, which helped him identify the optimal conditions for bacterial growth.

Case Study 2: Study of β-galactosidase Kinetics in Plant Tissues

Dr. Johnson, a plant biologist at the University of ABC, utilized 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 to study the kinetics of β-galactosidase in plant tissues. By analyzing the enzyme activity under different conditions, Dr. Johnson was able to gain insights into the regulation of β-galactosidase in plant metabolism.

Soluções

5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 offers several solutions for researchers in the field of enzyme research:

• High Specificity: The compound is highly specific for β-galactosidase, ensuring accurate and reliable results.
• High Sensitivity: The compound can be detected at low concentrations, allowing for sensitive measurements.
• Simple and Rapid Assays: The colorimetric assay using this compound is straightforward and can be performed quickly, saving time and resources.

Orientação especializada

When using 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5, it is essential to follow the expert guidance provided by the manufacturer. This includes proper handling, storage, and disposal of the compound, as well as the recommended assay protocols for accurate and reproducible results.

Conclusão

5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside CAS 107021-38-5 is a valuable tool for targeting enzyme activity in various research fields. Its high specificity, sensitivity, and ease of use make it an ideal choice for biochemical assays, enzyme kinetics studies, and protein engineering. By following the expert guidance and utilizing the compound effectively, researchers can achieve accurate and reproducible results in their studies.

Palavras-chave

5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside, β-galactosidase, enzyme activity, substrate, biochemical assay, enzyme kinetics, protein engineering

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