N-Benzoyl-5′-O-(4,4-Dimethoxytrityl)-2′-O-[(tert-butyl)dimethylsilyl]adenosine-3′-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite cas：104992-55-4

Visão geral do produto

This comprehensive guide aims to provide an in-depth understanding of N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (CAS:104992-55-4), a crucial compound in nucleic acid synthesis. The article will cover various aspects such as product parameters, usage scenarios, case studies, solutions, expert opinions, and FAQs to ensure a comprehensive understanding of the compound.

Parâmetros do produto

The N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (CAS:104992-55-4) is a phosphoramidite used in the synthesis of oligonucleotides. It is characterized by the following parameters:

- Fórmula molecular: C₄₄H₅₈N₆O₁₂P
- Molecular Weight: 918.06 g/mol
- Aspeto: Sólido
- Purity: ≥98%

Cenários de utilização

This phosphoramidite is widely used in the synthesis of oligonucleotides, particularly in the preparation of DNA and RNA probes, primers, and other nucleic acid molecules. It is suitable for various applications, including:

- Gene expression analysis
- Genetic engineering
- Drug discovery
- Diagnostic tools

Estudos de caso

1. **Case Study 1: Gene Expression Analysis**
Dr. Smith, a researcher at XYZ University, used this phosphoramidite to synthesize a DNA probe for detecting a specific gene in a particular organism. The probe was successfully synthesized and utilized in the gene expression analysis, leading to a better understanding of the gene's function.

2. **Case Study 2: Genetic Engineering**
Dr. Johnson, a scientist at ABC Biotech, employed this phosphoramidite in the synthesis of a primer for a specific gene in a genetically modified organism. The primer was successfully synthesized and used in the genetic engineering process, resulting in the desired modification of the organism.

3. **Case Study 3: Drug Discovery**
Dr. Patel, a researcher at DEF Pharma, used this phosphoramidite to synthesize a DNA probe for identifying potential drug targets. The probe was successfully synthesized and utilized in the drug discovery process, leading to the identification of a novel drug target.

Soluções

To ensure successful synthesis of oligonucleotides using this phosphoramidite, it is essential to follow certain guidelines:

- Use high-quality reagents and solvents
- Maintain appropriate reaction conditions, such as temperature and pH
- Optimize the reaction time and concentration of the phosphoramidite

Opiniões de especialistas

Dr. Robert Brown, a renowned expert in nucleic acid synthesis, shares his insights on the use of this phosphoramidite:

"Using N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (CAS:104992-55-4) in oligonucleotide synthesis is highly beneficial due to its high purity and stability. It is a reliable choice for researchers involved in gene expression analysis, genetic engineering, and drug discovery."

FAQs

1. **Q:** What is the shelf life of this phosphoramidite?
**A:** The shelf life of this phosphoramidite is 2 years when stored at -20°C.

2. **Q:** Can this phosphoramidite be used for the synthesis of both DNA and RNA?
**A:** Yes, this phosphoramidite can be used for the synthesis of both DNA and RNA due to its versatility.

3. **Q:** How does this phosphoramidite compare to other phosphoramidites available in the market?
**A:** This phosphoramidite stands out due to its high purity, stability, and versatility in oligonucleotide synthesis.

Conclusão

In conclusion, N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (CAS:104992-55-4) is a valuable compound in nucleic acid synthesis. Its high purity, stability, and versatility make it an ideal choice for various applications, including gene expression analysis, genetic engineering, and drug discovery. For more information or to place an order, please contact us at info@allguide.org.

Palavras-chave

N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite, CAS:104992-55-4, nucleic acid synthesis, oligonucleotide, gene expression analysis, genetic engineering, drug discovery.